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m vs. pH that indicated the deprotonation of a single group necessary for maximum activity ended up suit to equation three, where by c is the most activity and K
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The effects of solvent viscosity on the catalytic charge constants for your hydrolysis of 1 by Zn-NagA are shown in Figures 4A and 4B applying sucrose as the micro-viscogen. The plot of relative k
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this residue is hydrogen bonded for the lone h2o molecule (or hydroxide) that's coordinated towards the metallic ion sure to the Mβ-web-site (21). As a result, it is expected this residue will purpose in catalysis by abstraction of a proton from h2o just before, or concomitant with, the attack of hydroxide over the amide bond with the substrate. In the detection limitations of our assay for solution development, we ended up unable to evaluate any catalytic activity for both the D273N or D273A mutant.
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